Question

facts of hybridization

Answer 1

Hybridization is a technique in which molecules of single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) are bound tocomplementary sequences of either single-stranded DNA or RNA. Complementary base pairsare adenine (A) with thymine (T) or uracil (U) and vice versa, and guanine (G) with cytosine (C) and vice versa. Although the DNA double helix is relatively stable at body temperatures, high temperatures can split, or "melt," the double helix into single, complementary strands. After disrupting the double helix in this way, lowering the temperature then causes the single-stranded DNA to base-pair, or anneal, to other single strands that have complementary sequences.