Findout incorrect statement of the following wrt DNA replication
(A) DNA replication is initiated at a region called origin of replication
(B) In E.coli the rate of average polymerisation is 2000 bp per second
(C) Deoxy ribonucleotide triphosphates act as substrates and also
provide energy
(D) The main enzyme is DNA dependent DNA polymerase
Answers
(a). The origin of replication (also called the replication origin) is a particular sequence in a genome at which replication is initiated. ... This can either involve the replication of DNA in living organisms such as prokaryotes and eukaryotes, or that of DNA or RNA in viruses, such as double-stranded RNA viruses.
(b). Highly efficient to carry out polymerization of a large number of nucleotides in a very short time. The process of replication in E. coli, that has only 4.6 x 106 bp (base pairs) is completed within 18 minutes. This indicates that the average rate of polymerization is approximately 2000 bp per second.
(c). The Deoxyribonucleoside Triposphate act as both energy and substrate for the polymerisation reaction. ... The DTPs possess two high-energy phosphate that has the ability to provide energy to the replication by breaking their molecules. It serves as a substrate that is consumed during the enzymatic reaction of replication.
(d). DNA polymerase III holoenzyme is the primary enzyme involved in DNA replication in E. coli and belongs to family C polymerases. It consists of three assemblies: the pol III core, the beta sliding clamp processivity factor, and the clamp-loading complex.
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