For protein detection,most commonly used probe is
Interferon, Lectin, Antigen, Antibody
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interferon and antibody
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Detection of protein by the use of probe
Explanation:
- Most analysis ways, as well as western blotting and enzyme-linked Immunosorbent Assay (ELISA), that square measure designed to live the presence or amount of specific proteins or different molecules in biological samples depend upon the employment of target-specific probes that square measure detectable via chemical tags or labels. Antibodies square measure the foremost common variety of probe; their binding affinity for specific antigens change those targets to be "found" and detected during a advanced sample. However, antibodies square measure themselves proteins, and that they don't seem to be specifically detectable in Associate in Nursing assay system unless they're labeled for visual image or secondarily probed with another molecule that's labeled .
- Different types of chemical labels or tags are often conjugated to secondary or primary antibodies and different molecules to facilitate their visual image (i.e., detection and measurement) by numerous ways. Radioisotopes were used extensively within the past, however they're dear, have a brief shelf-life, provide no improvement in signal: noise magnitude relation and need special handling and disposal.
- Most used of probe is interferon.
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