Question

foreign DNA and plasmid were cut with two different restriction endonucleases. DNA ligase was then added to join the sticky ends of the DNA fragments. However the recombinant plasmid could not be formed. To rectify the mistake in the procedure one has to (A) Cut only the plasmid with restriction enzyme (B) Cut only the foreign DNA with restriction enzyme (C) Not use a plamid Cut the foreign DNA and plasmid with same the


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Answer 1

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Answer 2

Answer:

foreign DNA and plasmid were cut with two different restriction endonucleases. DNA ligase was then added to join the sticky ends of the DNA fragments. However the recombinant plasmid could not be formed. To rectify the mistake in the procedure one has to (A) Cut only the plasmid with restriction enzyme(B) Cut only the foreign DNA with restriction enzyme (C) Not use a plasmid Cut the foreign DNA and plasmid with same the

Explanation:

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