Question

Genetic engineering​

Answer 1

Answer:

Genetic engineering, is the direct manipulation of an organism's genes using biotechnology.

Answer 2

Genetic Engineering

• Agrobacterium tumefaciens is called genetic engineer.

• Because some part of its Ti plasmid called T-DNA is transferred and incorporated into host cell DNA where it is expressed along with the host DNA.

• Any gene contained in T-DNA therefore becomes part of host genetis system.

• If the plasmid in the bacterium does not have an insert, the presence of a chromogenic substrate gives blue coloured colonies.

• The presence of insert results into insertional inactivation of the beta-galactosidase and the colonies do not produce any colour.

• Such colonies are marked as recombinant colonies.