Question

Genetically engineered insulin can be obtained by
(a) recombinant DNA technique with the help
of E. coli
(b) two coded insulin genes separated then
incorporated into bacteria
(c) the extraction of cow's and pig's pancreas
(d) technique not developed till now

Answer 1

Answer:

ANSWER IS A

Explanation:

RECOMINANT DNA TECHNIQUE WITH THE HELP OF E COLI

Answer 2

(a) recombinant DNA technique with the help  of E. coli

Explanation:

A small piece of circular DNA called plasmid (autonomously replicating extra-chromosomal DNA) is extracted from E.coli bacteria or yeast cell. Through 'molecular scissors' so called restriction enzymes, a small section is cut out of the circular DNA (plasmid) at specific location.

The cut piece of DNA was then attached with the plasmid DNA and gene for human insulin is inserted into the circular DNA this is now genetically modified plasmid vector. This plasmid is introduced into a new bacteria which then divides rapidly and starts making insulin. This is the proinsulin which needs purification by deletion of c peptide and decontamination.