Question

Which one of the following bacteria has found
extensive use in genetic engineering work in
plants?
(a) Clostridium septicum
(b) Xanthomonas citri
(c) Bacillus coagulens
(d) Agrobacterium tumefaciens

Answer 1

Answer:

the the bacteria that is found extensive use in in genetic engineering work in plant is agrobacterium tumefaciens

Answer 2

Agrobacterium tumefaciens bacteria has found  extensive use in genetic engineering work in  plants.

Explanation:

• A bacterial plant pathogen that is soil borne and has tumorous growth is Agrobacteria .
• It has been widely used in the process of introducing genes that are foreign in nature to the plants.
• It also helps in the regeneration of the transgenic plants from the above process.
• Agrobacterium tumefaciens is a bacterium that has the ability of transferring a specific segment of DNA of the tumor inducing plasmid to the nucleus of the cell that is infected .
• It will undergo the stable integration with the host gene and this causes the crown gall disease.  

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Biotechnologists refer to Agrobacterium tumefaciens as a natural genetic engineer of plants. Give reasons to support the statement.

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What modification is done on Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning