Question

give a short note on the process of tissue culture​

Answer 1

Answer:

Plant Tissue culture has a great significance in plant biotechnology, especially in the crop improvement programme's. The term tissue culture may be defined as the process of the in-vitro culture of explants in a nutrient medium under aseptic conditions. However, in general, the tissue culture includes the term tissue culture as well as cell culture, organ culture and suspension culture also. Totipotency is the genetic potential of a plant cell to produce the entire plant. In other words, totipotency is the cell characteristics in which the potential for forming all the cell types in the adult organism is retained. The basis of tissue culture is to grow a large number of cells in a sterile controlled environment. The cells are obtained from stem, root or other plant parts and are allowed to grow in culture medium containing mineral nutrients, vitamins and hormones to encourage cell division and growth. As a result, the cells in culture will produce an unorganised proliferative mass of cells which is known as callus tissue.

⚫BASIC REQUIREMENTS AND TECHNIQUES OF PLANT TISSUE CULTURE:-

The main requirements of plant Tissue culture are:-

1) Laboratory organization

2) Culture Media

3) Aseptic Condition

Advantage of plant Tissue culture:-

1. it can create a large number of clones from a single SEED or ex plants

2. it takes shortened time, no need to wait for the whole life cycle of seed development.

3) It overcomes seasonal restriction for seed germination.

4)it helps to eliminate the plant disease through The careful stock solution and sterile technique.

Answer 2

Explanation:

Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. Plant tissue culture is widely used to produce clones of a plant in a method known as micropropagation.

Tissue culture is a technique in which small tissue pieces or organs are removed from a donor plant and cultured aseptically on a nutrient medium.