Question

Give an account of amplification of gene of interest using PCR ? Help in simple way

Answer 1

Polymerase Chain reaction (PCR) is used for amplicfication of any gene of your interest. PCR works on the principle of Denaturation, Annealing and Extension. As the sequence of your gene of interest is known Primers which are short oligonucleotide sequences are designed which are complementary to your gene of interest. During denaturation the template DNA which your provide..