Question

Give the process of agrobacterium mediated hairy root culture.

Answer 1

This is the answer see the attachment bro

Answer 2

Hairy Root Cultures

When Agrobacterium rhizogenes, a Gram-negative soil bacterium, infects plants, adventitious roots called ‘hairy roots’ are induced from the infected site.110,111 This event occurs due to the transfer of the particular DNA region called transfer DNA (T-DNA) comprising the loci between the TR and TL regions of the root-inducing (Ri) plasmid of the bacterium into the plant genome. The basic molecular mechanism of T-DNA trimming from the Ri plasmid, transfer to plant cells, and integration into the plant genome is known, although the functions of several genes on the T-DNA have not yet been elucidated. The hairy roots are aseptically cultured in vitro without added phytohormones.