give two examples of staining agent with their reaction
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diazine green(Janus green B), iodine, saffrin etc.. are some staining agents....
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Examples of staining agent:
Staining is the process by which a substance like a tissue or a polymer is coloured to some contrasting colour by means of adding different chemicals, which makes it easier to study at cellular level.
Stains include:
- Leishman stain – it is eosinated methylene blue in acetone-free methyl alcohol. Eosin is acidic part of the stain which reacts with basic part and methylene blue is basic part that reacts with acidic part.
- Gram stain – it is staining of acid-fast bacteria with methyl violet, also called gentian violet. This is a basic dye, which reacts with acidic bacteria to give a blue colour.
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