Glycolysis pathway substrate level phosphorylation
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Glycolysis (Glycos Sugar,Lysis Splitting)Breakdown of glucose to pyruvic acid by partial oxidation.Scheme given by Embden, Meyerhof and Parnas.Common pathway for aerobic and anaerobic cellular respiration.Occurs in the cytoplasm of a cell.Present in all living organisms.In plants, sucrose is converted into glucose.Hexokinase: Enzyme that phosphorylates glucose to produce glucose 6 phosphate.2 ATPs are utilised in two steps:
/>Glucose→Glucose 6 phosphate (1 ATP)
Fructose 6 phosphate→fructose 1, 6 bisphosphate (1 ATP) Fructose 1,6 bisphosphate splits into glyceraldehyde 3 phosphate and dihydroxy acetone phosphate.Glyceraldehyde 3 phosphate convertsinto two molecules of 1, 3 bisphosphoglycerate (BPGA), with subsequent conversion of NAD+to NADH + H+.4 ATPs are yielded in two steps: BPGA→PGA (1×2 = 2 ATPs)Phosphoenol pyruvate→Pyruvic acid (1×2 =2 ATPs) Net ATPs produced = 4 (produced) 2 (consumed) = 2 ATPs
Fructose 6 phosphate→fructose 1, 6 bisphosphate (1 ATP) Fructose 1,6 bisphosphate splits into glyceraldehyde 3 phosphate and dihydroxy acetone phosphate.Glyceraldehyde 3 phosphate convertsinto two molecules of 1, 3 bisphosphoglycerate (BPGA), with subsequent conversion of NAD+to NADH + H+.4 ATPs are yielded in two steps: BPGA→PGA (1×2 = 2 ATPs)Phosphoenol pyruvate→Pyruvic acid (1×2 =2 ATPs) Net ATPs produced = 4 (produced) 2 (consumed) = 2 ATPs
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