Hi i would like to know if anyone tried ChIP of a specifc loci and performed a mass spec on the endogenous proteins bound to that loci?
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If yes, please mention how much cells you used and what is the protein concentration requirement for mass spec, what tags are better to use for the pulldown. For example: I am trying to analyze all the proteins bound to a specific gene promoter under 2 different conditions.
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good morning dear what's up
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