Hloo guys......
Draw the primary,secondary, tertiary and quaternary structure of proteins!
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The primary structure of protein is the hierarchy's basic level, and is the particular linear sequence of amino acids comprising one polypeptide chain. ... Quaternary structure is the next 'step up' between two or more polypeptide chains from the tertiary structure and is the specific spatial arrangement and interactions.
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Heating proteins causes them to become denatured, which means losing their higher order (secondary, tertiary, and quaternary) structure. The primary structure (linear sequence of amino acids) usually remains intact if the heating is applied at near-neutral pH because the amide bonds between amino acids are quite stable under those conditions.
Proteins can differ markedly in their sensitivities to heat denaturation. Some are stable at 100oC, whereas other denature at much lower temperatures. Highly thermostable proteins tend to be richer in stabilizing hydrogen bonds and salt bridges and have more compact structure than less thermostable proteins.
It is important to remember that higher-order protein structure is ultimately determined (mainly) by the primary structure, so the thermostability is also determined, ultimately, by the primary structure.
Heating double-stranded DNA causes it to denature, also. In this case, denaturation means separation of the two strands of the double helix. The thermostability of DNA is determined by the sequence of nucleotide bases. G-C base pairs have three hydrogen bonds, whereas A-T base pairs have only two. Therefore, the thermostability increases as the proportion of G-C base pairs increases.
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