Question

how can check transformed and untransformed bacterial cells
if both are kanamycine resistance

Answer 1

hey!!
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▪Steps of bacterial transformation and selection.

▪Here is a typical procedure for transforming and selecting bacteria:-

▪The bacteria are given a heat shock.
which encourages them to take up a plasmid.

▪ Most bacteria do not take up a plasmid, but some do.

▪Plasmids used in cloning contain an antibiotic resistance gene.

▪Thus, all of the bacteria are placed on an antibiotic plate to select for ones that took up a plasmid.

▪Bacteria without a plasmid die. Each bacterium with a plasmid gives rise to a cluster of identical plasmid containing bacteria called a colony.

▪A typical colony looks like a small whitish dot the size of a pinhead.

▪Several colonies are checked to identify one with the right plasmid.

▪A colony containing the right plasmid is grown in bulk and used for plasmid or protein production.


▪The bacteria are given a heat shock, which causes some of them to take up a plasmid.
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Q.How does that work?
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Ans:-
Plasmids used in cloning contain an antibiotic resistance gene.

▪Thus, all of the bacteria are placed on an antibiotic plate to select for ones that took up a plasmid.

hope help.u !
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Answer 2

Answer:

itni biology kisne pdaaa di sir mujhe bhi pdaa do