Question

How can we decontaminate bacterial contamination from cell line?

Answer 1

Answer:

I guess first thing to do is to autoclave the incubator, make fresh medium (use freshly made antibiotic). Clean the laminar hood, including below, have the filter checked. Autoclaving the pipet you use for cell culture if they are autoclavable is also a good idea. A clean environment is essential, otherwise even if you cure the cells the contamination will return (particularly important is to have the hood efficiency checked)

And then start a fresh culture, it is very hard to get rid of bacteria completely and the danger is that low level contamination affects the results of experiment (particularly ROS studies will not give reproducible results).