Question

How can we select transformants and recombinants using selectable markers?

Answer 1

Answer:

A selectable marker is a reporter gene introduced into a cell along with a gene insert. What this means is, the experimenter can tell the right gene is in the cell because the marker can be seen or detected. Most often, this is used for bacteria or for cells in culture.

In the Simple Cloning Lab, transformants are E. coli clones, harbouring a pGT4 plasmid, or a pGT4 derived plasmid. By a heatshock, the plasmids has been transferred into competent bacterial cells. As a result cells are ampicillin resistant, so they can be selected by growing on the antibiotic ampicillin.

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