How can we use LEU 2 gene as a selectable marker?
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High impact information on LEU2
Nucleotide sequence of yeast LEU2 shows 5'-noncodingregion has sequences cognate to leucine [6].
Recombinant DNA procedures and the yeasttransformation technique were used to insert the yeastgene LEU 2 (coding for beta-isopropylmalatedehydrogenase) into the tandem array of ribosomal DNAgenes of the yeast Saccharomyces cerevisiae [7].
Role of an upstream regulatory element in leucinerepression of the Saccharomyces cerevisiae leu2 gene [8].
This element is located within a 280 base pair (bp)fragment which occurs 125 bp upstream of the leu2translation initiation codon and which contains a short G +C-rich palindromic sequence [8].
Southern-type hybridization to DNAs isolated from thetransformed yeast clones revealed that the yeast plasmidcarrying the prokaryotic methylase gene, as well as thetwo chromosomal genes tested (his3 and leu2) weremethylated, whereas the bulk of the yeast DNA remainedlargely unmethylated
Nucleotide sequence of yeast LEU2 shows 5'-noncodingregion has sequences cognate to leucine [6].
Recombinant DNA procedures and the yeasttransformation technique were used to insert the yeastgene LEU 2 (coding for beta-isopropylmalatedehydrogenase) into the tandem array of ribosomal DNAgenes of the yeast Saccharomyces cerevisiae [7].
Role of an upstream regulatory element in leucinerepression of the Saccharomyces cerevisiae leu2 gene [8].
This element is located within a 280 base pair (bp)fragment which occurs 125 bp upstream of the leu2translation initiation codon and which contains a short G +C-rich palindromic sequence [8].
Southern-type hybridization to DNAs isolated from thetransformed yeast clones revealed that the yeast plasmidcarrying the prokaryotic methylase gene, as well as thetwo chromosomal genes tested (his3 and leu2) weremethylated, whereas the bulk of the yeast DNA remainedlargely unmethylated
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