How does ion exchange chromatography work?
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Principle of Ion Exchange Chromatography. IEX chromatography is used in the separation of charged biomolecules. The crude sample containing charged molecules is used as the liquid phase. When it passes through the chromatographic column, molecules bind to oppositely charged sites in the stationary phase.
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Principles of Ion Exchange Chromatography. The most popular method for the purification of proteins and other charged molecules is ion exchange chromatography. In cation exchange chromatography positively charged molecules are attracted to a negatively charged solid support
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