Question

How does restriction endonuclease function 2?

Answer 1

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 restriction endonuclease, also called Restriction enzyme, a protein produced by bacteria that cleaves DNA at specific sites along the molecule. In the bacterial cell, restriction enzymescleave foreign DNA, thus eliminating infecting organisms.

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Answer 2

Restriction Endonuclease

》The enzyme which cuts the DNA strand at the ends is known as restriction endonuclease.

》This enzyme functions by recognising its recognition sites of the DNA sequence.

》Once it find its recognition site, it binds to the DNA and cuts the two opposite strands.

》These recognition sites are known as palindromic sequence.

》It cuts the DNA strand away from the palindromic sequence.

》The opposite strands of the DNA are cut between the same two base pairs which results in the formation of overhanging stretches called Sticky Ends.