Question

How does the initiation of translation in eukaryotes differ from that in
e. coli?

Answer 1

in prokaryotes transcription and translation are clubbed process since it's nucleus does not have a membrane. Therefore most of the time you might find a prokaryotic DNA being transcribed and the newly formed RNA being translated simultaneously.
On the other hand in eukaryotes the RNA formed inside the nucleus needs to be transported to cytoplasm for translation.
Also rRNA which is the ribosome- factor for protein synthesis are different in both. Prokaryotes have 70s and Eukaryotes have 80s ribosome. Also the large subunit of the ribosome in Eukaryotes have Peptide (P)site, Acceptor(A) site and Exit(E) site whereas in prokaryotes E site is absent.
Translation is a major question in your boards as well as competitive exams.
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