How does UV visible spectroscopy work?
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Ultraviolet and visible (UV-Vis) absorption spectroscopy is the measurement of the attenuation of a beam of light after it passes through a sample or after reflection from a sample surface. Absorption measurements can be at a single wavelength or over an extended spectral range.
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In a cuvette substance is taken and then UV vis Spectrum of different wavelengths are passed or dropped on this sample . And then amount of wavelength and its range is noted .
IF no absorbtion takes place than transmittance is 100%
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