How error prone PCR can be run?
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Error-prone PCR (EP-PCR) is the method of choice for introducing random mutations into a defined segment of DNA that is too long to be chemically synthesized as a degenerate sequence (UNIT 8.2A).
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Error-prone PCR. Error prone PCR is a method by which random mutants maybe inserted into any piece of DNA. ... In these conditions, the polymerase makes mistakes in the base paring during DNA synthesis that results in the introduction of errors in the newly synthesized complementary DNA strand.
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