How fluorescein isothiocyanate used in immunofluorescence?
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Explanation:
Immunofluorescence (IF) or cell imaging techniques rely on the use of antibodies to label a specific target antigen with a fluorescent dye (also called fluorophores or fluorochromes) such as fluorescein isothiocyanate (FITC). ... Direct IF uses a single antibody directed against the target of interest.
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fluorescein is an fluorescent due involved in attachment to a specific antigen. This labelling is done with the help of antibodies.
Now with the help of fluorescent microscope, the target distribution can be analysed and cell imaging is possible.
This is it's use in immunofluorescence.
Hope you will be able to understand.
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