How is repetitive / satellite DNA separated from bulk genomic DNA for various genetic experiments?
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Density-gradient centrifugation is the technique used for the separation of satellite DNA from bulk genomic DNA for various genetic experiments.
• Most of the satellite DNA consists of repeated nucleotide sequences or short DNA sequences.
• These are separated by density gradient centrifugation method.
• Density-gradient centrifugation is invented by the scientists Myron Brakke.
• In this technique, the DNA particles are separated based on the rate of sedimentation.
• The sedimentation rate depends upon the particle size, shape and thickness.
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Repetitive/satellite DNA separation
Explanation:
- There are regions in a DNA sequence called repetitive DNA on the grounds that these successions have a little stretch of DNA that is rehashed commonly.
- These monotonous/satellite DNA are isolated from mass genomic DNA in a type of various tops by the procedure of thickness angle centrifugation.
- The mass DNA right now a significant pinnacle and the other little pinnacles shaped are called satellite DNA.
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