How is the amplification of a gene sample of interest carried out using Polymerase Chain Reaction (PCR)?
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Polymerase chain reaction (PCR) is the process in which the amplification of the gene of interest is carried out with two sets of primers and a thermostable DNA polymerase enzyme Taq polymerase. ... Annealing: The two sets of primers are added which bind to the appropriate complementary segment of DNA strand at 54 0C.
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Polymerase chain reaction (PCR) is a method widely used in molecular biology to rapidly make millions to billions of copies of a specific DNA sample allowing scientists to take a very small sample of DNA and amplify it to a large enough amount to study in detail. PCR was invented in 1983 by Kary Mullis.
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