Question

How many basepairs of dna are there in one mammalian cell?

Answer 1

Answer:

That mass would actual depend on the cytogenetic status, i.e. ploidity, of the cell or line. This status may even vary with prolonged culture time and is therefore quite inconsistently reported in the literature for most lines. However, on average, that may not matter a lot to you. Human lines, like your HT1080 fibrosarcoma cells or esp. the HeLa adenocarcinoma cells, are often hyperploid. If you know the number of base pairs in the genome (e.g. COS-7 = monkey!) of your cell of interest, which is about 3 billion in the haploid human genome, you may calculate as follows: 3x10^9 bp x 2 (diploid) x 660 (AVGed MW of 1 bp) x 1.67x10^-12pg ("weight in dalton") = 6.6pg/ diploid primary cell. Without having own experiences with Hoechst 33342, you may test this empirically by using single cells from the different lines with different ploidities. Hope this helps. Good luck!

Explanation:

Answer 2

$3.3 \times {10}^{9} \: bp$

are there in one mammalian cell