How the Plants can be transformed using agro-bacterium mediated Plant transformation? Step by step process, Discuss in details?
Answers
Answer:
The Agrobacterium-mediated transformation process involves a number of steps:
Explanation:
=>(a) isolation of the genes of interest from the source plant ; (b) development of a functional transgenic construct including the gene of interest; promoters to drive expression; codon modification, if needed to increase successful protein production; and marker genes to facilitate tracking of the introduced genes in the host plant; (c) insertion of the transgene into the Ti-plasmid; (d) introduction of the T-DNA-containing-plasmid into Agrobacterium; (e) mixture of the transformed Agrobacterium with plant cells to allow transfer of T-DNA into plant chromosome; (f) regeneration of the transformed cells into genetically modified (GM) plants; and (g) testing for trait performance or transgene expression at lab, greenhouse and field level. I had given Figure to illustrates Agrobacterium-mediated plant transformation. .
Please thanks me if you like my answer and give me 5 star
