How the restriction endonuclease cut the recognition sequence?
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Restriction Endonuclease
》The enzyme which cuts the DNA strand at the ends is known as restriction endonuclease.
》They have specific recognition sites on the DNA strands characterized by some specific base pairs.
》It first suspects the entire strand of the DNA and once it find its recognition site, it binds to the DNA and cuts the two opposite strands.
》These recognition sites are known as palindromic sequence.
》It cuts the DNA strand a little away from the palindromic sequence.
》It cuts the opposite strands between the same two base pairs which results in the formation of overhanging stretches called Sticky Ends.
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