Question

How to calculate km and vmax from raw data?

Answer 1

Vmax is the maximum rate of an enzyme catalysed reaction i.e. when the enzyme is saturated by the substrate. Km is measure of how easily the enzyme can be saturated by the substrate. 


For each substrate concentration, calculate the rate (velocity) of reaction (Absorbance units produced per unit Time).


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Answer 2

When the enzyme is saturated by the waste substance, Vmax can be used to measure the maximum rate of an enzyme catalysed reaction.

On the other part, Km can be used to measure the saturated rate of an enzyme by the waste substances.

Calculate the rate or velocity of reaction units produced per time from the raw data.