How to detect magnesium phosphate from polymerase chain reaction?
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The basics of Magnesiums function in PCR. MgCl2 acts as a cofactor and is a catalyzer in PCR. That means, higher concentrations of MgCl2 increases higher productivity of Taq polymerase. But the specificity will be less with high productivity and causes ugly band smears in your gel.
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It is used to the magnesium phosphate the reaction..
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