How to get rid of optical reflections in microscopy?
Answers
Answered by
0
We are working on reflective DHM and the system looks like the setup in figure 1. We are facing a severe problem of reflection in the system.
Apart from the reference beam and object beam, there is a unknown strong spot on the CCD. In figure 2, the black bar is the object, the bright concentric spot is the reflection of the objective lens (if a plano-convex replaces the objective, the bright spot still exists). When the distance between the objective and condenser changes, the radius of the spot changes as well. We guess it originates from this lens, but we have no idea how to remove it.
In addition, in figure 3, the condenser is placed above the BS and the reference beam is blocked. The bright concentric spot changes to interference pattern. Does anybody have any clue on this problem? Thanks.
Apart from the reference beam and object beam, there is a unknown strong spot on the CCD. In figure 2, the black bar is the object, the bright concentric spot is the reflection of the objective lens (if a plano-convex replaces the objective, the bright spot still exists). When the distance between the objective and condenser changes, the radius of the spot changes as well. We guess it originates from this lens, but we have no idea how to remove it.
In addition, in figure 3, the condenser is placed above the BS and the reference beam is blocked. The bright concentric spot changes to interference pattern. Does anybody have any clue on this problem? Thanks.
Answered by
0
Deconvolution is a technique to get rid of this out-of-focus information by applying a mathematical algorithm.
Similar questions