Question

How to preparation dna stock by using citrate buffer?

Answer 1

Citrate Buffer (0.1 M, pH 6.0) recipe and preparation

Prepare 800 mL of distilled water in a suitable container.

Add 12.044 g of Sodium Citrate dihydrate to the solution.

Add 11.341 g of Citric Acid to the solution.

Adjust solution to desired pH using 0.1N HCl (typically pH ≈ 6.0).

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