Question

How to prepare 0.01m sodium borate buffer?

Answer 1

sodium phosphate dibasic heptahydrate (or 28.4 g of the anhydrous form) 1 L dH20 0.1 M Buffer To make buffer, combine the following quantities of each stock and add dH2O to bring up to 600 ml: monobasic dibasic pH (ml) (ml) 280.5 19.5 5.7 276 24 5.8 270 30 5.9 263.1 36.9 6.0 255 45 6.1 244.5 55.5 6.2 232.5 67.5 6.3 220.5 79.5 6.4 205.5 94.5 6.5 187.5 112.5 6.6 169.5 130.5 6.7 153 147 6.8 135 165 6.9 117 183 7.0 99 201 7.1 84 216 7.2 69 231 7.3 57 243 7.4 48 252 7.5 39 261 7.6 31.5 271.5 7.7 25.5 274.5 7.8 21 279 7.9 15.9 284.1 8.0