Question

How to preserve purified recombinant proteins for long time?

Answer 1

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▶Methods are:-
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[1] Freezing samples in liquid nitrogen: adjust pH and salt concentration, it depends on your protein. add 5%- 20% glycerol to your sample before you freeze them in liequid nitrogen and then store them at -80°C.

[2] Adjust pH and salt concentration and add to 50% glycerol, store samples at -20°C directly (don't freeze them in liquid N2).

[3] Lyophylization. and store at 4°C or -20°C.

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Answer 2

Answer:

If any protein encoding gene is expressed in a heltrologus host it is called recombinant protein.

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