HOW TO PURIFY THE PROTEIN OF INTEREST IF ITS THE SAME SIZE AS THE CONTAMINATING PROTEIN?
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Purified proteins are required for many experimental applications, including structural studies and in vitro biochemical assays. Proteins can be obtained from a tissue or, more often, by their overexpression in a model organism, such as bacteria, yeast, or mammalian cells in culture. Protein purification involves isolating proteins from the source, based on differences in their physical properties. The objective of a protein purification scheme is to retain the largest amount of the functional protein with fewest contaminants. The purification scheme of a protein must be optimized to complete this process in the least number of steps.
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