Question

How to seperate bacteria from gut of fish without culturing?

Answer 1

ANSWER-:

Firstly, there is no single method that will isolate all bacteria from such a sample. Many of the bacteria in question may be obligate anaerobes, while others may be strictly aerobic. Secondly, to identify all bacteria is a different question from identifying culturable bacteria. The gut microbiome is complex and still not completely understood. I suggest collaborating with someone who is an expert in gut microbial ecology - there are many out there