How to seperate bacteria from gut of fish without culturing?
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Firstly, there is no single method that will isolate all bacteria from such a sample. Many of the bacteria in question may be obligate anaerobes, while others may be strictly aerobic. Secondly, to identify all bacteria is a different question from identifying culturable bacteria. The gut microbiome is complex and still not completely understood. I suggest collaborating with someone who is an expert in gut microbial ecology - there are many out there
Firstly, there is no single method that will isolate all bacteria from such a sample. Many of the bacteria in question may be obligate anaerobes, while others may be strictly aerobic. Secondly, to identify all bacteria is a different question from identifying culturable bacteria. The gut microbiome is complex and still not completely understood. I suggest collaborating with someone who is an expert in gut microbial ecology - there are many out there
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