Question

how transferment can be differentiated from nontransforment by insertional inactivation of a galactoside gene​

Answer 1

Answer:

Alternative selectable markers have been developed which differentiate recombinants from the non-recombinants on the basis of their ability to produce colour in the presence of a chromogenic substrate. In this, a recombinant DNA is inserted within the coding sequence of an enzyme, galactosidase. This results into inactivation of the enzyme, which is referred to as insertional inactivation. The presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert. Presence of insert results into insertional inactivation of the -galactosidase gene and the colonies do not produce any colour, these are identified as recombinant colonies.

So, the correct answer is 'A recombinant DNA is inserted within the coding sequence of enzyme -galactosidase, resulting in inactivation of the enzyme'