how will you detect the presence of protein in a given food sample.
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Proteins are detected using Biuret reagent . This turns a mauve or purple colour when mixed with protein.
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Following steps are used to test if a food item contains protein. 1) Take a small quantity of the food items to be tested.2) Grind/mash the food item.3) Put the food item into a test tube.4) Add 10 drops of water and shake the test tube.5) Add two drops of copper sulphate solution to the test tube, using a dropper. 6) Add 10 drops of caustic soda solution using a dropper and shake the test tube.7) Let the test tube rest in a test tube stand for a few minutes.8) Observe the colour of the mixture.9) Violet colour indicates the presence of proteins in the tested food item.
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