Question

how will you prepared a temporary slide of onion scale peel​

Answer 1

• Take a medium sized onion, cut its outer surface with knife.

• Use forceps to remove the peel of onion.

• With the help of needle separate the small portion of epidermis (peel)

• Keep dilute safranin solution in a watch glass.

• Put this small peel in this watch glass with brush and allow it to stain for 3-5 minutes.

• Transfer the stained peel to another watch glass that contains distilled water in it, to remove extra stain.

• Take a clean dry slide and place two drops of water/glycerine on the centre of the slide.

• Transfer the stained peel with needle and brush on the middle of the slide, if the peel curls straighten it and flatten it with brush and needle, do this gently.

• With the help of blade cut the peel into a square shape.

• Take a dry and clean coverslip and gently place it on the slide with the help of needle such that no air bubbles enter in it.

• Gently press the coverslip with needle for even spreading of glycerine.

• Remove the extra stain and water with the help of blotting paper.

• Clean the sides of the coverslip with dry blotting paper and place it under the lens of the microscope and record your observations.

HOPE IT HELPS YOU

Answer 2

Answer:

Experimental Preparation of Temporary Mounts of an Onion Peel !

                                                Experiment:

Objective:

To prepare a stained temporary mount of an onion peel and to record observations and draw labeled diagrams.

Apparatus and materials required:

An onion, glass slide, watch glass, coverslip, forceps, needles, brush, blade, filter paper, safranin, glycerine, dropper, water, and a compound microscope.

Procedure:

      Take an onion and remove its outermost peel.

• Now cut a small part from an inner scale leaf with the help of a blade.
• Separate a thin, transparent peel from the convex surface of the scale leaf with the help of forceps.
•  Keep this peel in a watch glass containing water?
•  Add two drops of safranin stain in the watch glass to stain the peel.
• . Take a clean slide and put a drop of glycerine in the centre of the slide.
•  With the help of a brush and needle transfer the peel on the slide. Glycerine prevents the peel from drying up.
•  Carefully cover it with a coverslip and avoid any air bubble from entering interring the coverslip.
• Remove any excessive glycerine with a filter paper.

 Observe the prepared mount of the peel under the low and high magnification of a compound microscope.

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