(I) explain why the investigation was carried out at 40 degrees
(II) using the information in fig 3.2 (Q9) describe the effect of pH on the rate of enzyme activity
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Answer: Because most animal enzymes rapidly become denatured at temperatures above 40°C, most enzyme determinations are carried out somewhat below that temperature. Over a period of time, enzymes will be deactivated at even moderate temperatures.
Core practical - Investigating the effect of pH on enzyme...
Set up a Bunsen burner, heatproof mat, tripod and gauze.
Place a beaker of water on the gauze and adjust the flame to keep the water at about 35°C.
Now put two drops of iodine solution into each spot of a spotting tile.
Add 2 cm 3 of amylase enzyme solution to a test tube.
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Answer:
cause 40c is the optimum temperature for mammal enzymes
To control a variable to make the investigation measuring PH only
Explanation:
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