Question

If bacteria grows / divides from
1x 10^4 to 6.5 x 10^7 in a hours Calculate
for 8 hours ?​

Answer 1

Answer:

First thing we need to know is the Dilution Factor, or how much the original sample is diluted:

here Initially 5mL in 45mL = Final Volume / Sample volume = 50/5 = 10.

Then two serial dilutions of 1/100.

Total Dilution Factor = 10 * 100 *100 = 10^5

CFU/mL = cfu/ml = (no. of colonies x dilution factor) / volume of culture plate

= (137 * 10^5)/0.1

=1.37*10^8

So Total colony forming units = 1.37*10^8 CFU/mL

Converting CFU/mL to Log value

For example,

Total colony forming units = 1.37*10^8 CFU/mL and you want to convert it into Log value,

Just take Log(CFU/mL)

Here, log (1.37*10^8) = 8.13924922.