If DNA is digested by endonucleases in four sites giving rise to fragments of which two are equal in length
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If DNA is digested by endonucleases in four sites giving rise to fragments of which two are equal in length how many bands would be seen after electrophoresis? Explanation: Digestion at four sites gives rise to five fragments. Two fragments are of same size thus they will form a single band
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If DNA is linear then the number of fragments generated is (N+1), where N= number of recognition sites or sequences. Hence the number of fragments generated is 5 if we digest a linear DNA molecule with a restriction enzyme having four recognition sites on the DNA.
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