Question

If the serum is removed from human kidney cell lines what will happen

Answer 1

Normal animal tissues (e.g., skin, kidney, liver) or whole embryos commonly are used to establish primary cell cultures. To prepare tissue cells for culture (or to remove adherent cells from a culture dish for biochemical studies), trypsin or another protease is used to destroy the proteins in the junctions that normally interconnect cells. For many years, most cell types were difficult, if not impossible, to culture. But the identification and preparation of various protein growth factors that stimulate the replication of specific cell types, as well as other recent modifications in culture methods, now permit experimenters to grow various types of specialized cells.

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