If you are given a sample of a tissue
to study it in subcellular (organelles)
level what strategy would you
adopt and why?
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Explanation:
Separation of cellular compartments from one another is an important step for studying a specific intracellular structure or organelle or protein, or to assess possible associations between these macromolecular structures. Subcellular fractionation uses one or more of the properties of each compartment, such as buoyant density, surface charge density, size and shape, and is mainly based on differential centrifugation in media of high viscosity at 4°C.
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