Question

If you are provided with a purified protein with one additional contaminant protein which has a different size, what strategy you would adopt when you see that size exclusion column analysis of the protein solution came up with only one peak?​

Answer 1

Answer:

The size exclusion chromatogram shows a major Gaussian-shaped peak with an elution volume corresponding to a protein-detergent complex with an apparent molecular weight (MW) of 126 kDa. A void volume peak suggested that the Ni-NTA purified sample contained sizable amounts of aggregated protein

Explanation: