Question

If you have a restriction enzyme that cuts a piece of DNA at two recognition sites, how many DNA fragments would you see on a gel?

Answer 1

E-Gel CloneWell II agarose gels are double-comb gels with a twist. Load your sample into the top row and electrophorese until your band migrates into the bottom row (Figure 1). Then simply pipet out your purified DNA band and you’re ready to clone. That’s it. No additional purification kits or steps are required. Use the Invitrogen E-Gel Power Snap System, a compact, self-contained device with a built-in power supply and blue-light transilluminator, to run and visualize E-Gel CloneWell II agarose gels.

Three easy steps for separation and isolation of DNA bands with E-Gel CloneWell II agarose gels

Figure 1. Three easy steps for separation and isolation of DNA bands with E-Gel CloneWell II agarose gels. Samples are loaded into the top row of wells, bands separate during the gel run, and individual bands are collected from the bottom row as they enter those wells. Reverse-run functionality on the E-Gel Power Snap System lets you capture bands of interest even if you miss the bands when they pass through the collection wells.

Answer 2

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Answer:

3 Fragments

Explanation:

YY is your restriction site.

ABCD-YY-ABCDE-YY-ABCDEF

When you cut, you'd get

ABCD-Y

Y-ABCDE-Y

Y-ABCDEF

HOPE IT HELPS YOU !!