Question

illustrate the steps for selective enrichment of vibrio cholerae​

Answer 1

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-Inoculate a single colony of V. cholerae into 5 ml liquid LB (see Basic Protocol 2). ...

-Incubate at 37°C shaking for 15-16 hr.

-Remove 1 ml of bacterial culture.

-Add 1 ml of culture to 1.5 ml 50% glycerol solution.

-Place suspension into a freezer vial.

Mix well by vortexing.

-Immediately place into -80°C freezer.

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Answer 2

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☆What is vibrio cholerae?

It is a bacteria which causes cholera.

Cholera: Cholera is an infectious disease that causes severe watery diarrhea, which can lead to dehydration and even death if untreated.

☆Selective enrichment of vibrio cholerae:

Thiosulfate-citrate-bile salts-sucrose agar, or TCBS agar, is a type of selective agar culture plate that is used in microbiology laboratories to isolate Vibrio species.