Question

In an elisa you might use an antigen or antibody labeled with

Answer 1

125-Iodine

Explanation:

Answer 2

In an ELISA you might use an antigen or antibody labeled with a reporter enzyme or a tag that reacts directly with the antigen.

• The plate-based assay method known as ELISA (enzyme-linked immunosorbent assay) is used to identify and measure soluble molecules such as peptides, proteins, antibodies, and hormones.
• The same method is often referred to by other names, like enzyme immunoassay (EIA). An ELISA involves immobilizing the target macromolecule (antigen) on a solid surface (microplate) and then combining it with an antibody that is connected to a reporting enzyme.
• By incubating the reporter enzyme with the proper substrate to produce a quantifiable end product, detection is carried out. An ELISA's most important component is a highly selective antibody-antigen interaction.
• A primary antibody labeled with a reporter enzyme or a tag that reacts directly with the antigen is used in the direct detection technique.
• Direct detection can be carried out using a capture assay format or an antigen that is directly immobilized on the assay plate.