In order to isolate genes for cloning into another organism, dna is frequently fragmented by enzymes. In order to purify these fragments, what is done next?
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Gel Electrophoresis is used to seperate fragments according to their size.
• Agarose gel filled medium contains numerous micropores.
• Fragmented DNA is put in to it and electric field is applied.
DNA being negatively charged it moves towards positive end through the pores of agarose gel.
• As it moves through it , it gets seperated according to size.
• Then the regions were DNA are selerated is cut out from gel and is stained using ethydium bromide. Passing UV light makes it appear brown. Hence it can be identified.
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